Ocean-optics OOISensors Uživatelský manuál

► OOISensors Fiber Optic Sensors System Installation and Operation Manual Document Number FOXY-AL300-000-02-0207 Offices: Ocean Opti

Table of Contents Product-Related Documentation • MultiFrequency Phase Fluorometer Installation and Operation Manuall • Various USB spectrometer d

6: Hardware Data Sheets and Instructions Oxygen Probe Temperature Control Accessories Temperature affects the fluorescence decay time, the fluoresce

6: Hardware Data Sheets and Instructions 6. Locate the port on the module that is connected to the thermistor in use. Note The RS-232 module can s

6: Hardware Data Sheets and Instructions Operation ► Procedure 1. Connect the FOXY-TK1 to the 10-foot wire (model number FOXY-T-WIRE) that comes w

6: Hardware Data Sheets and Instructions Specification Value Data acquisition FOXY-T-MOD-K RS-232 module Probe material Chomega-Alomega 304 stainl

6: Hardware Data Sheets and Instructions Specification Value Element type Platinum 100 ohm Single 3 Wire Sheath material 316 SS Light Sources The

6: Hardware Data Sheets and Instructions Using the Continuous Mode ► Procedure 1. Turn the switch on the back of the LS-450 to Contin. This means

6: Hardware Data Sheets and Instructions • When using the pulsed flash mode, you need to ensure that a constant number of flashes occur for every

6: Hardware Data Sheets and Instructions R-LS-450 Rack Mount Blue LED Pulsed Light Source The R-LS-450 is shipped with the following jumper pin co

6: Hardware Data Sheets and Instructions Note This feature is only available with an ADC1000 A/D converter and with a J-series or later version of t

6: Hardware Data Sheets and Instructions R-LS-450 Operating Matrix The following table provides information on configuring the jumper blocks on the

Chapter 1 Sensors System Overview Overview The following sections provide a general overview of the Oxygen Sensors System and the pH Sensors System.

6: Hardware Data Sheets and Instructions • The switch is turned to pulsed mode • There is a jumper over the pins in JP1 of the R-LS-450 • There is a

6: Hardware Data Sheets and Instructions Pins on the JP3 (of the S2000) Integration time for DAQ700 (multiple of) Integration time for SAD500 (multi

6: Hardware Data Sheets and Instructions USB-LS-450 Temperature Measurement Specifications Specification Value Precision Better than 0.1° C Accurac

6: Hardware Data Sheets and Instructions Use Notes • Gently remove the plastic cover from the SMA connector before use. Pulling the SMA connector aw

6: Hardware Data Sheets and Instructions FOXY/HOXY/FOSPOR General Specifications Specification FOXY HIOXY FOSPOR Principle Photoluminescence-quenc

6: Hardware Data Sheets and Instructions FOSPOR Specifications Parameter Dissolved Oxygen in Liquid Oxygen Gas (at 1 atmosphere) Sensor mechanism

6: Hardware Data Sheets and Instructions FOXY/MFPF Specifications Specification Dissolved Oxygen in H2O Oxygen Gas (at 1 atmosphere) Sensor mechan

6: Hardware Data Sheets and Instructions HIOXY/MFPF Specifications Specification Dissolved Oxygen in Liquid Oxygen Gas (at 1 atmosphere) Sensor me

6: Hardware Data Sheets and Instructions 108 FOXY-AL300-000-02-0207

Chapter 7 Troubleshooting Overview The following sections contain information that may assist you in troubleshooting some of the more common problems

1: Sensors System Overview FOXY Probes FOXY oxygen sensors are designed for monitoring oxygen partial pressure in gas and aqueous solutions. Stand

7: Troubleshooting Issue Possible Cause Resolution Peaks displayed in the graph screen are in incorrect places. Wavelength coefficients may be incor

7: Troubleshooting Issue Possible Cause Resolution High response times when measuring Samples Sample condition may be adversely affecting response

7: Troubleshooting Remove the Unknown Device from Windows Device Manager ► Procedure 1. Open Windows Device Manager. Consult the Windows operating

7: Troubleshooting 2. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows 2000 or XP operating system, you m

7: Troubleshooting 114 FOXY-AL300-000-02-0207

Appendix A External Triggering Ocean Optics S2000-series spectrometers, when used with OOISensors Software, provide two methods of acquiring data: no

A: External Triggering 116 FOXY-AL300-000-02-0207

Appendix B Chemical Effects on Oxygen Probes Summary of Chemical Effects on Oxygen Probes (Data Available upon Request) Chemicals Known to be Harmfu

B: Chemical Effects on Oxygen Probes Chemical Supporting Data Available Gasoline (even in headspace) Yes N-Vinyl-2-Pyrrolidinone (even at 2.5%) Ye

Appendix C pH Sensor Theory of Operation and Calculations Overview The following sections contain the equations that express the theory of pH sensor

1: Sensors System Overview Hardware The following hardware is included with the Fiber Optic Sensors System or is compatible with the Fiber Optic Sen

C: pH Sensor Theory of Operation and Calculations Model # 1 Looks at base peak only. No baseline correction. Uses nominal pK and slope. HighpHAb

C: ph Sensor Theory of Operation and Calculations correct for baseline aselineAbsLowBufferBAAbsLowBufferAbsLowBufferA−=−−][][ sBaselineAbHighBuffer

C: pH Sensor Theory of Operation and Calculations Model # 4 In addition to previous, it uses both acid and base peaks LowBufferpHLowH−=10 HighBuffer

C: ph Sensor Theory of Operation and Calculations Revised Model #4 Looks at base peak only. Corrects for baseline. lineAbsHighpHBaseAsBaselineAb

C: pH Sensor Theory of Operation and Calculations 124 FOXY-AL300-000-02-0207

Index A Accessories, 66 Bifurcated Optical Fiber Assemblies, 71 Overcoats, 68 Probes, 66 Sol-Gel Coated Membranes, 69 Temperature Compensation, 69 Au

Index F Factory Calibration, 48 Factory Calibration Services, 88 File Menu, 31 Exit, 32 Open Spectrum, 32 Open Time Chart, 32 Page Setup, 32 Print S

Index P Packing List, 4 Page Setup, 32 pH, 1 Films, 58 pH Probes, 58 RFP200-UV-VIS Reflective Film Probe, 59 TP300-UV-VIS Probe, 58 pH Sensors, 58 C

Index 128 FOXY-AL300-000-02-0207

1: Sensors System Overview Documentation The Fiber Optic Sensors System contains various documentation components on paper, floppy diskette and CD

Chapter 2 OOISensors Software OOISensors Overview OOISensors Software is Ocean Optics’ next generation of operating software for the Fiber Optic Sens

2: OOISensors Software Installing OOISensors Before installing OOISensors Software, make sure that no other applications are running. ► Procedu

2: OOISensors Software 3. Click Next at the Welcome screen. The Choose Destination Location screen appears. 4. Accept the default or select another

2: OOISensors Software Configuring OOISensors Software Once OOISensors is installed and the system has been rebooted, connect the spectrometer to t

2: OOISensors Software Configure Hardware The Configure | Hardware dialog box allows you to specify the hardware parameters for the spectrometer. Ty

Additional Offices: Ocean Optics Asia 137 Xianxia Road, Suite 1802, Changning District, Shanghai, PRC. 200051Phone 86.21.5206.8686 Fax 86.21.

2: OOISensors Software If you choose the ADC1000-USB A/D Converter: Specify the serial number of the ADC1000-USB, or select the First Available ADC

2: OOISensors Software The System Type field in the upper, right corner allows you to select either Spectrometer or MFPF for the attached hardware.

2: OOISensors Software Sensors Tab for Spectrometer Systems Click on the Sensors tab in the Configure System screen to access Sensor controls. The

2: OOISensors Software Option Name Description Choose a spectrometer channel to view its sensor parameters Selects the spectrometer channel for wh

2: OOISensors Software Option Name Description Pressure Compensation CH Monitors and corrects for pressure fluctuations in the sample (if you have

2: OOISensors Software Option Name Description Temperature Measurement Specifies the method used to monitor and correct for temperature fluctuation

2: OOISensors Software pH Use this screen to set parameters for a pH sensor. 16 FOXY-AL300-000-02-0207

2: OOISensors Software Pressure Use this screen to set parameters for a pressure sensor. FOXY-AL300-000-02-0207 17

2: OOISensors Software CO2 Use this screen to enter parameters for a CO2 sensor. Timing Tab Click on the Timing tab in the Configure System screen

2: OOISensors Software The Timing tab contains the following options: Option Name Description Preset Duration Specifies the length of the entire t

Table of Contents About This Manual ... 7 Doc

2: OOISensors Software Option Name Description External Software Trigger: This mode allows data acquisition to be synchronized with external event

2: OOISensors Software Option Name Description Spectral Graph & Time Chart: The Spectral Graph appears in the top of the display window and the

2: OOISensors Software The Log tab contains the following options: Option Name Description Store to disk every X acquisitions Specifies the numbe

2: OOISensors Software ► Procedure 1. Select the Single or Cont. button from the Scan section of the OOISensors screen in the lower right hand corn

2: OOISensors Software OOISensors always subtracts the dark measurement from the sample measurement in the OOISensors algorithm. Data Acquisition P

2: OOISensors Software Average This parameter specifies the number of spectra to average for the specified spectrometer channel. A higher value for

2: OOISensors Software Magnification Options There are several magnification options from which to choose. The function chosen remains in use until

2: OOISensors Software X and Y Values The cursor taskbar displays the X value and Y value of the cursor point. Cursor Properties To the right of t

2: OOISensors Software Temperature Chart The Temperature Chart, when enabled, displays in the upper half of the OOISensors main screen. When this o

2: OOISensors Software ► Procedure To save the Temperature Chart without displaying the chart: 1. Select Configure from the OOISensors menu bar. 2

Table of Contents Sensors Tab for Spectrometer Systems ... 11 pH ...

2: OOISensors Software Log On/Off Switch You can enable or disable the saving of data to a log file with the Log switch. Click on the switch to ena

2: OOISensors Software Save Time Chart This menu option saves the current time chart as a tab-delimited ASCII file. You can then open these files as

2: OOISensors Software Clear Spectrum Graph This option removes static spectra overlays from the graph. Clear Time Chart This option clears the ti

2: OOISensors Software Scan This option takes a scan of your sample. When in Single mode, this function acts as a snapshot. When the button is dep

2: OOISensors Software Calibrate Menu Functions The following section provides information on options available from the Spectrometer menu. Optio

2: OOISensors Software Notes Be sure to retain the Wavelength Calibration Data. You will need this information if you reinstall OOISensors. If you

2: OOISensors Software 36 FOXY-AL300-000-02-0207

Chapter 3 Oxygen Sensors The following section provides information on operation of the oxygen sensors, as well as the theory behind sensor operati

3: Oxygen Sensors Fluorescence Quenching The following paragraph explains the principle of fluorescence quenching as it relates to oxygen sensors. O

3: Oxygen Sensors Calibration Requirements for Oxygen Sensors The following section explains the principles of calibration for oxygen sensors, as we

Table of Contents Chapter 3: Oxygen Sensors ...37 How the Oxygen Sensors Work ...

3: Oxygen Sensors The Stern-Volmer constant (k) is primarily dependent on the chemical composition of the ruthenium complex. Ocean Optics probes hav

3: Oxygen Sensors Compensating for Temperature with the Second Order Polynomial Algorithm If you decide to compensate for temperature, the relation

3: Oxygen Sensors T (ºC) T* (T/100K) Mole Fraction of oxygen in water at 1 atmosphere pO2 Weight Fraction (ppm) at 1 atmosphere pO2 (pure O2)

3: Oxygen Sensors Samples should be maintained at a constant (+/-3° C*) temperature. If this is not practical, calibrate the Fiber Optic Sensors Sys

3: Oxygen Sensors Calibrating Oxygen Sensors The following sections provide instructions on calibrating your oxygen sensors both with and without te

3: Oxygen Sensors 9. Select the algorithm type from the Curve Fitting drop-down menu. This algorithm will be used to calibrate your sensor system.

3: Oxygen Sensors 5. Once you click the green Scan Standard button, a red Scanning button appears. Watch the values in the Intensity column. When t

3: Oxygen Sensors Calibrating Oxygen Sensors with Temperature Compensation Perform the steps below to calibrate your oxygen sensors using temperatur

3: Oxygen Sensors 3. Adjust the integration time so that the fluorescence peak does not exceed 3500 counts. Set the integration time to powers of t

3: Oxygen Sensors 6. Get the proper temperature reading. Either: • Select Spectrometer | Sample Temperature from the menu bar in the Single Tempera

Table of Contents Sol-Gel Coated Membranes, Microscope Coverslips, and Slides ... 68 Spectrometers ...

3: Oxygen Sensors Note If the probe has an overcoat, then the standards used in the calibration can be of a state of matter different from the sampl

3: Oxygen Sensors 2. Set the integration time for the entire calibration procedure when the probe is measuring the standard with zero concentration

3: Oxygen Sensors 4. Place your cursor under the oxygen concentration of the first standard. 5. Click the green Scan Standard button or select S

3: Oxygen Sensors Finalizing Calibration without Using Factory Calibration – Part 1 ► Procedure 1. Click the green Curve Fit button. A graph displ

3: Oxygen Sensors Note In order to use the Second Order Polynomial algorithm, you must have had at least three standards of known oxygen concentrati

3: Oxygen Sensors 6. Click the green Scan Standard button or select Spectrometer | Scan Standard from the menu bar. Note Enabling the optional Cont

3: Oxygen Sensors • chXFoxy.cal The OOISensors.cfg file is the application configuration file. Calibration data is called from this binary file each

Chapter 4 pH Sensors Overview The Fiber Optic pH Sensor system consists of a fiber optic probe designed to hold immobilized indicator dye materials

4: pH Sensors Note You can also use an RT-2-10 or RT-10-20 transflection tip, which enables the TP300-UV-VIS to be used for routine transmission m

4: pH Sensors 2. Activate channels with pH sensors and enter their analysis wavelengths (table on next page): • HA (acid peak) • A (base peak) • B

Table of Contents FOXY-RESP Specifications ... 86 Factory

4: pH Sensors Note Be careful not to over-tighten the tip. Over-tightening the tip can break the mirror in the transmission probe or break the film

4: pH Sensors Calibration The following sections contain information on calibrating your Fiber Optic pH Sensor system. Calibration requires recordin

4: pH Sensors • Integration Period, Average, and Boxcar Smoothing are displayed for the active channel. These values can be modified and will be us

4: pH Sensors 9. Click on the Update Channel Calibration button. • From this point on, when you click the Scan button, OOISensors displays intens

4: pH Sensors 64 FOXY-AL300-000-02-0207

Chapter 5 Compatible Products and Accessories Overview The following sections provide ordering information for products and accessories compatible

5: Compatible Products and Accessories Part Number Description FOXY-OR125-GT 1000-µm core diameter titanium fiber optic probe, 1/8" outer diam

5: Compatible Products and Accessories Part Number Description HIOXY-AL300 300-µm fiber probe in aluminum jacketing, 1m HIOXY-OR125 1000-µm fib

5: Compatible Products and Accessories Part Number Description FOXY-AF RTV healthcare-grade silicone overcoat for oxygen probes FOXY-AF-MG RTV hi

5: Compatible Products and Accessories Part Number Description FOXY-GF Sol-gel coated glass fiber membranes (pack of 5) for testing viability of

Table of Contents Appendix B: Chemical Effects on Oxygen Probes ...117 Summary of Chemical Effects on Oxygen Probes ...

5: Compatible Products and Accessories Bifurcated Optical Fiber Assemblies and Splice Bushing http://www.oceanoptics.com/products/premgradebif.aspOc

Chapter 6 Hardware Datasheets and Instructions The following section contains product data and operating instructions for hardware that is specific

6: Hardware Data Sheets and Instructions FOXY Probes Probe Construction The standard oxygen probe has its distal tip polished and coated with the o

6: Hardware Data Sheets and Instructions Sampling Chambers The oxygen probe should be installed in a suitable sampling compartment. If you are not u

6: Hardware Data Sheets and Instructions Temperature and the Oxygen Probe Temperature affects the fluorescence decay time, the fluorescence intensit

6: Hardware Data Sheets and Instructions Use Warnings • Handle with care. Dropping the probe may cause permanent damage. • Gently remove the plastic

6: Hardware Data Sheets and Instructions Method Results ETO (at room temperature) Unknown. ETO (at temperatures above 100 °C) Unknown. Hydrogen Pe

6: Hardware Data Sheets and Instructions FOXY-R Probe FOXY-R Specifications Specification Value Fiber core 1000 µm core silica Fiber cladding Sili

6: Hardware Data Sheets and Instructions FOXY-AL300 Aluminum-jacketed Fiber Optic Probe The FOXY-AL300 is a 300-µm aluminum jacketed fiber optic pro

6: Hardware Data Sheets and Instructions Specification Value Maximum applied pressure 300 psi Probe temperature range -80° C to 80° C (reflects ran

About This Manual Document Purpose and Intended Audience This document provides you with an installation and configuration instructions to get your

6: Hardware Data Sheets and Instructions FOXY-PI600 Specifications Specification Value Fiber core 600 µm silica Fiber coating 25 µm polyimide Outer

6: Hardware Data Sheets and Instructions Foxy 21G (left) and 18G (right) Fiber Optic Probe FOXY-18G/21G Specifications Specification Value Fiber c

6: Hardware Data Sheets and Instructions FOXY-OR125 and FOXY-OR125G O-ring Fiber Optic Probes The FOXY-OR125 is a silica-core, 1000-µm stainless ste

6: Hardware Data Sheets and Instructions Specification Value Probe temperature range -80° C to 80° C (reflects range of ruthenium complex in the sol

6: Hardware Data Sheets and Instructions FOXY-T1000 Specifications Specification Value Fiber core 1000 µm silica Fiber cladding Silica Fiber jacket

6: Hardware Data Sheets and Instructions FOXY-T1000-RTD Stainless Steel Probe with Light Shield FOXY-T1000-RTD Specifications Specification Value

6: Hardware Data Sheets and Instructions FOXY-RESP Respiration Probe The FOXY-RESP Respiration Probe is typically used for measuring oxygen tension

6: Hardware Data Sheets and Instructions Specification Value Compensation Temperature Probe reconditioning N/A – disposable sensor films Re-calibra

6: Hardware Data Sheets and Instructions Sample spectra indicative of a compromised probe overcoat FOXY-AF and FOXY-AF-MG Silicone Overcoats The FO

6: Hardware Data Sheets and Instructions Sterilizing See the Sterilization table in the Oxygen Sensing Probes – General Information section of this